Journal: Clinical and Experimental Vaccine Research
Article Title: Immunogenicity and efficacy of a plasmid DNA rabies vaccine incorporating Myd88 as a genetic adjuvant
doi: 10.7774/cevr.2014.3.2.202
Figure Lengend Snippet: Gel image showing polymerase chain reaction amplification of rabies virus glycoprotein gene. Lane 1, lambda DNA/ Eco RI+ Hind III marker; lane 2, ~1.6 kb band indicative of full-length glycoprotein gene.
Article Snippet: Polymerase chain reaction (PCR) reagents [ Pfu DNA polymerase (Cat. No. EP0571), dNTP mix (Cat. No. R1121)], T4 DNA ligase (Cat. No. EL0014), TurboFect in vitro Transfection Reagent (Cat. No. R0531), Lambda DNA/ Eco RI+ Hind III marker (Cat. No. SM0193), GeneRuler 100 bp DNA ladder (Cat. No. SM0241) and 6× DNA loading dye (Cat. No. R0611) were purchased from Thermo Scientific (Waltham, MA, USA).
Techniques: Polymerase Chain Reaction, Amplification, Virus, Lambda DNA Preparation, Marker